Объем | 50 мкг |
Синонимы | Peroxiredoxin-6 (EC 1.11.1.15) (1-Cys peroxiredoxin) (1-Cys PRX) (24 kDa protein) (Acidic calcium-independent phospholipase A2) (aiPLA2) (EC 3.1.1.4) (Antioxidant protein 2) (Liver 2D page spot 40) (Non-selenium glutathione peroxidase) (NSGPx) (Red blood cells page spot 12), PRDX6, AOP2 KIAA0106 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P30041 |
Иммуноген | Recombinant Human Peroxiredoxin-6 protein (87-224AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
Примечание | Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Peroxiredoxin-6 |
Области исследований | Cancer, Cardiovascular, Cell biology, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate
All lanes: PRDX6 antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA018659LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human glioma using CSB-PA018659LA01HU at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA018659LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating PRDX6 in Hela whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA018659LA01HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA018659LA01HU (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
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