PRKAA1 (Ab-174/172) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | AMPK, AMPKa1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q13131 | | Иммуноген | Peptide sequence around aa. 172~176/170~174 (L-R-T-S-C) derived from Human AMPKa1/AMPKa2. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 | | Примечание | Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. <br/><br/>Hurley RL, et al. (2005) J Biol Chem. Aug 12, 280(32): 29060-29066<br/>Woods A, et al. (2003) Curr Biol. Nov 11, 13(22): 2004-2008<br/>Nielsen JN, et al. (2003) J Appl Physiol. Feb, 94(2): 631-641<br/>Da Silva Xavier G, et al. (2000) Proc Natl Acad Sci U S A. Apr 11, 97(8): 4023-4028.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Neuroscience,Cancer,Cardiovascular,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from A549, HepG2 and 3T3 cells using AMPKa1/AMPKa2(Ab-174/172) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AMPKa1/AMPKa2(Ab-174/172) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 | Immunofluorescence staining of methanol-fixed Hela cells using AMPKa1/AMPKa2(Ab-174/172) Antibody.
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Наименование: PRKAA1 (Ab-174/172) Antibody.