| Объем | 100 мкл |
| Синонимы | CDC25M2, MPIP2, Dual specificity phosphatase Cdc25B, CDC25HU2, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P30305 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 353(R-R-S(p)-V-T) derived from Human CDC25B . |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB,WB:1:500-1:1000 |
| Примечание | <p>Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. <br/><br/>Reboutier D, et al. (2012) J Cell Biol 197, 19-26 <br />Lobjois V, et al. (2011) Biochem Biophys Res Commun 410, 87-90 <br />Feng C, et al. (2007) Biol Reprod 77, 560-8 </p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser353 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from COS7 cells treated with Etoposide using Phospho-CDC25B (Ser353) antibody.The lane on the right is treated with the antigen-specific peptide.
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Наименование: Phospho-CDC25B (Ser353) Antibody.