| Объем | 100 мкг |
| Синонимы | Prohibitin, PHB |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P35232 |
| Иммуноген | Recombinant Human Prohibitin protein (1-272AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:200-1:1000, IHC:1:10-1:100, IP:1:200-1:2000 |
| Примечание | Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology, Tags & Cell Markers, Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Prohibitin antibody at 2µg/ml + Mouse brain tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 30, 18 kDa
Observed band size: 30 kDa
 |
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA017885LA01HU at dilution of 1:20
 |
Immunoprecipitating PHB in HEK293 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA017885LA01HU in HEK293 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA017885LA01HU (8µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (10µg)
 |
Western Blot
Positive WB detected in: Raji whole cell lysate
All lanes: PHB antibody at 4.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30, 18 kDa
Observed band size: 30 kDa
 |
Immunoprecipitating PHB in HEK293 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA017885LA01HU in HEK293 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA017885LA01HU (8µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (10µg)
 |
| | |
Наименование: PHB Antibody.