| Объем | 100 мкл |
| Синонимы | KC1A, CKI-alpha, CSNK1A1, alpha isoform, Casein kinase I |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P48729/Q8N752 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y(p)-T-F) derived from Human CK-1alpha. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.<br/><br/>Tapia C., FEBS Lett. 349:307-312(1994).<br />Fish K.J., J. Biol. Chem. 270:14875-14883(1995).<br />Halleck A., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr294 |
| Области исследований | Signal transduction,Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HT-29 cells treated with heat shock using CK-1? (Phospho-Tyr294) Antibody.The lane on the right is treated with the antigen-specific peptide.
 |
Immunohistochemical analysis of paraffin-embedded human brain tissue using CK-1? (Phospho-Tyr294) antibody (left)or the same antibody preincubated with blocking peptide (right).
 |
| | |
Наименование: Phospho-CSNK1A1/CSNK1A1L (Tyr294) Antibody.