| Объем | 100 мкл |
| Синонимы | ADRB2, E2AK2, EIF2AK2, EIF2aK, PRKR |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P19525 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine 446 (K-R-T(p)-R-S) derived from Human PKR. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. <br /><br />Abujiang Pataer, et,al. (2002) Cancer Res, 62: 2239. <br />K. D. Ryman, et,al. (2005) J. Virol, 79: 1487 - 1499. <br />Susana Guerra, et,al. (2006) J. Biol. Chem, 281: 18734 - 18745. <br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr446 |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from K562 cells using PKR(Phospho-Thr446) Antibody(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKR(Phospho-Thr446) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using PKR(Phospho-Thr446) Antibody.
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Наименование: Phospho-EIF2AK2 (Thr446) Antibody.