| Объем | 100 мкл |
| Синонимы | BP-1, 4EBP1, 4E-BP1, PHAS-I, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13541 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine 36(S-T-T(p)-P-G) derived from Human 4E-BP1. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p> ,Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr36 |
| Области исследований | Epigenetics and Nuclear Signaling,Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from MDA-MB-435 cells, untreated or EGF-treated (200 ng/ml, 30min), using 4E-BP1 (Ab-36) antibody (Lane 1 and 2) and 4E-BP1 (phospho-Thr36) antibody (Lane 3 and 4).
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (phospho-Thr36) antibody.
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Наименование: Phospho-EIF4EBP1 (Thr36) Antibody.