| Объем | 100 мкл |
| Синонимы | 4EBP1, P/OKCL.6, P/OKCL.6, PHAS-I, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13541 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine70 (T-K-T(p)-P-P) derived from Human 4E-BP1. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, IHC,IHC:1:50-1:100 |
| Примечание | <p>4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.<br/><br/>Pause A., Nature 371:762-767(1994).<br />Gevaert K., Nat. Biotechnol. 21:566-569(2003).<br />Haghighat A., EMBO J. 14:5701-5709(1995).</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr70 |
| Области исследований | Epigenetics and Nuclear Signaling,Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Phospho-Thr70) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Наименование: Phospho-EIF4EBP1 (Thr70) Antibody.