| Объем | 100 мкл |
| Синонимы | CREB2, CREBP1, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P15336 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine 73 or 55 (T-P-T(p)-R-F) derived from Human ATF2. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat, Monkey |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex. <br /><br />Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135. <br />Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. <br />Gupta S, et al. (1995) Science. 267: 389-393. <br />Van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811. <br />Livingstone C, et al. (1995) EMBO J. 14: 1785-1797.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr73 or 55 |
| Области исследований | Epigenetics and Nuclear Signaling,Immunology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using ATF2(Phospho-Thr71 or 53) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Thr73 or 55) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using ATF2(Phospho-Thr73 or 55) Antibody.
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Western blot analysis of extracts from COS7 cells, treated with UV or calf intestinal phosphatase (CIP), using ATF2 (Phospho-Thr73 or 55) Antibody.
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Наименование: Phospho-ATF2 (Thr73 or 55) Antibody.