| Объем | 100 мкл |
| Синонимы | ATF4, CREB-2, DNA-binding protein TAXREB67, TXREB, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P18848 |
| Иммуноген | Peptide sequence around phosphorylation site of Serine 219 (N-D-S(p)-G-T) derived from Human ATF-4. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB,WB:1:500-1:1000 |
| Примечание | <p>ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA bindingprotein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.<br/><br/>Sato T., Shimotohno K. J. Virol. 65:1420-1426(1991)<br />Leiden J.M. Proc. Natl. Acad. Sci. U.S.A. 89:4820-4824(1992)<br />Bridgeman A.M. Wright H. Nature 402:489-495(1999)<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser219 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from COLO cells using ATF-4 (Phospho-Ser219) Antibody.The lane on the right is treated with the antigen-specific peptide.
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Наименование: Phospho-ATF4 (Ser219) Antibody.