| Объем | 100 мкл |
| Синонимы | C/ATF, C/EBP-related ATF, CREB2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P18848 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 245 (N-R-S(p)-L-P) derived from Human ATF4. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA bindingprotein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.<br/><br/>Yang X, et al. (2004). Cell.117(3): 387-398. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser245 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells untreated or treated with UV using ATF4(Phospho-Ser245) Antibody.
 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF4(Phospho-Ser245) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 |
| | |
Наименование: Phospho-ATF4 (Ser245) Antibody.