Объем | 100 мкл |
Синонимы | Histone H2AX, H2a/x, Histone H2A.X, H2AFX, H2AX |
Тип антител | Recombinant Antibody |
Species | Human |
UniProt ID | P16104 |
Иммуноген | A synthesized peptide |
Видовая специфичность | Human |
Применение | ELISA, WB, Recommended dilution: WB:1:3000-1:10000 |
Клональность | Monoclonal |
Изотип | Rabbit IgG |
Коньюгат | Non-conjugated |
Буффер | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Affinity-chromatography |
Области исследований | Epigenetics and Nuclear Signaling |
Аббревиатура | Histone H2AX |
Примечание | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. |
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Western Blot
Positive WB detected in:293 whole cell lysate
All lanes:Phospho-Histone H2AX (S139) antibody at 0.23µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 KDa
Observed band size: 15 KDa
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