Объем | 100 мкл |
Синонимы | HSTF 1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q00613 |
Иммуноген | Peptide sequence around phosphorylation site of serine 307 (P-Q-S(p)-P-R) derived from Human HSF1. |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IF,WB:1:500-1:1000, IF:1:100-1:200 |
Примечание | <p>DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. <br /><br />Guettouche T, et al. (2005) BMC Biochem , 6(1): 4 <br />Wang X, et al. (2003) Mol Cell Biol , 23(17): 6013-6026 <br />Jolly C, et al. (1999) Proc Natl Acad Sci USA, 96(12): 6769-6774.</p> |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Абревеатура | Phospho-Ser307 |
Области исследований | Epigenetics and Nuclear Signaling,Cardiovascular,Tags & Cell Markers |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HUVEC cells untreated(lane 1) or treated with TNF-a(lane 2) using HSF1(Phospho-Ser307) Antibody.
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Immunofluorescence staining of methanol-fixed Hela cells using HSF1(Phospho-Ser307) Antibody.
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Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using HSF1 (Phospho-Ser307) Antibody.
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