Объем | 100 мкл |
Синонимы | HS90B, HSP 84, HSP90-beta, HSP90AB1, HSPC2 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P08238 |
Иммуноген | Peptide sequence around phosphorylation site of serine 254 (V-G-S(p)-D-E) derived from Human HSP90B. |
Источник | Rabbit |
Видовая специфичность | Human, Mouse, Rat |
Применение | ELISA, WB,WB:1:500-1:1000 |
Примечание | <p>Molecular chaperone. Has ATPase activity. <br /><br />Zhao R, Houry WA.(2007) Adv Exp Med Biol, 594: 27-36. <br />Olsen JV, et al. (2006) Cell, 127(3): 635-648 <br />Ogiso H, et al. (2004) Biochemistry, 43(49): 15510-15519</p> |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Абревеатура | Phospho-Ser254 |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with hot shock(lane 2) using HSP90B(Phospho-Ser254) Antibody.
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Western blot analysis of extracts from Hela cells, treated with calf intestinal phosphatase (CIP), using HSP90B (Phospho-Ser254) Antibody.
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