| Объем | 100 мкл |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P14635 |
| Иммуноген | Peptide sequence around phosphorylation site of Serine 147 (A-F-S(p)-D-V) derived from Human Cyclin B1. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.<br/><br/>Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470.<br/>Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685.<br/>Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser147 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from MDA cells untreated or treated with Anisomycin using Cyclin B1(phospho-Ser147) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1(Phospho-Ser147) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using Cyclin B1(phospho-Ser147) Antibody.
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Наименование: Phospho-CCNB1 (Ser147) Antibody.