| Объем | 100 мкл |
| Синонимы | LIMK-2, kinase LIMK2, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P53671 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine 505 (R-Y-T(p)-V-V) derived from Human LIMK2. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.<br/><br/>Soosairajah J, et al. (2005) EMBO J.<br/>Ohashi K, et al. (2000) J Biol Chem, 275(5): 3577-82.<br/>Edwards DC, et al. (1999) Nat Cell Biol, 1(5): 253-9.<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr505 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells untreated or treated with PMA using LIMK2(Phospho-Thr505) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK2(Phospho-Thr505) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using LIMK2(Phospho-Thr505) Antibody.
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Наименование: Phospho-LIMK2 (Thr505) Antibody.