| Объем | 100 мкл |
| Синонимы | Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, hIRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, Endoribonuclease, ERN1 |
| Тип антител | Recombinant Antibody |
| Species | Human |
| UniProt ID | O75460 |
| Иммуноген | A synthesized peptide derived from human Phospho-ERN1 (S724) |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200 |
| Клональность | Monoclonal |
| Изотип | Rabbit IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Affinity-chromatography |
| Области исследований | Signal Transduction |
| Аббревиатура | Serine/threonine-protein kinase/endoribonuclease IRE1 |
| Примечание | Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation (PubMed:9637683, PubMed:11175748, PubMed:12637535). The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis (PubMed:24508390). |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in:293 whole cell lysate,A549 whole cell lysate,Hela whole cell lysate(treated with Calyculin A or EGF)
All lanes:Phospho-ERN1 antibody at 0.75µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 110 KDa
Observed band size: 110 KDa
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Immunofluorescence staining of Hela cells with CSB-RA007795A724phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Наименование: Phospho-ERN1 (S724) Antibody.