Объем | 50 мкл |
Синонимы | PDZ and LIM domain protein 1 (C-terminal LIM domain protein 1) (Elfin) (LIM domain protein CLP-36), PDLIM1, CLIM1 CLP36 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | O00151 |
Иммуноген | Recombinant Human PDZ and LIM domain protein 1 protein (1-329AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
Примечание | Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Области исследований | Epigenetics and Nuclear Signaling, Cancer, Metabolism |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: PDZ and LIM domain protein 1 antibody at 10µg/ml + Mouse lung tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA017731ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA017731ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA017731ESR2HU at dilution of 1:100
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Immunoprecipitating PDLIM1 in HepG2 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA017731ESR2HU in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA017731ESR2HU (4µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (20µg)
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