| Объем | 100 мкл |
| Синонимы | ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q02750 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 221 (A-N-S(p)-F-V) derived from Human MEK1. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. <br /><br />Zebisch A, et al. (2006) Cancer Res, 66(7): 3401-8. <br />Luciano BS, et al. (2006)J Biol Chem, 279(50): 52117-23. <br />Wang X, et al. (2003) Oncogene, 22(1): 109-16. <br />Gopalbhai K, et al. (2003) J Biol Chem, 278(10): 8118-25. <br />Ling MT, et al. (2002)Oncogene, 21(55): 8498-505.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser221 |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells untreated or treated with EGF using MEK1(Phospho-Ser221) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1(Phospho-Ser221) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using MEK1(Phospho-Ser221) Antibody.
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Western blot analysis of extracts from Hela cells, treated with calf intestinal phosphatase (CIP), using MEK1 (Phospho-Ser221) Antibody.
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Наименование: Phospho-MAP2K1 (Ser221) Antibody.