| Объем | 100 мкл |
| Синонимы | ATP synthase-coupling factor 6, mitochondrial (ATPase subunit F6), ATP5J, ATP5A ATPM |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P18859 |
| Иммуноген | Recombinant Human ATP synthase-coupling factor 6, mitochondrial protein (1-108AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 |
| Примечание | Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Cancer, Tags & Cell Markers, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: ATP5J antibody at 7µg/ml
Lane 1: Rat brain tissue
Lane 2: Mouse heart tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 13, 14 kDa
Observed band size: 13 kDa
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA002369ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA002369ESR1HU at dilution of 1:100
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Наименование: ATP5J Antibody.