Объем | 100 мкг |
Синонимы | V-type proton ATPase subunit E 2 (V-ATPase subunit E 2) (Vacuolar proton pump subunit E 2), ATP6V1E2, ATP6E1 ATP6EL2 ATP6V1EL2 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q96A05 |
Иммуноген | Recombinant Human V-type proton ATPase subunit E 2 protein (1-226AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, IF, Recommended dilution: IF:1:50-1:200 |
Примечание | Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform is essential for energy coupling involved in acidification of acrosome (By similarity). |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Cancer, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Immunofluorescence staining of HepG2 cells with CSB-PA836175LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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