| Объем | 100 мкл |
| Синонимы | HSTF 1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q00613 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 303 (P-P-S(p)-P-P) derived from Human HSF1. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. <br /><br />Parvaneh Rafiee, et,al. (2006) Am J Physiol Cell Physiol , 291: C931 - C945 <br />Fumika Shinozaki, et,al. (2006) J. Biol. Chem , 281: 16361 - 16369. <br />Eiichi Takaki, et,al. (2006) J. Biol. Chem , 281: 4931 - 4937. <br />Jan-Jong Hung, et,al. (1998) J. Biol. Chem , 273: 31924.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser303 |
| Области исследований | Epigenetics and Nuclear Signaling,Cardiovascular,Tags & Cell Markers |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from MCF7 cells using HSF1(phospho-Ser303) Antibody(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1(Phospho-Ser303) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using HSF1 (phospho-Ser303) Antibody.
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Наименование: Phospho-HSF1 (Ser303) Antibody.