Объем | 100 мкл |
Синонимы | AP-1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P17275 |
Иммуноген | Peptide sequence around phosphorylation site of serine 259 (P-V-S(p)-P-I) derived from Human JunB. |
Источник | Rabbit |
Видовая специфичность | Human, Mouse, Rat |
Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
Примечание | Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.<br/><br/>Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Абревеатура | Phospho-Ser259 |
Области исследований | Epigenetics and Nuclear Signaling,Cancer |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HT29 cells untreated or treated with EGF using JunB(Phospho-Ser259) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunB(Phospho-Ser259) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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