| Объем | 100 мкл |
| Синонимы | PLD 2, PLD1C, choline phosphatase 2, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O14939 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 169(E-N-Y(p)-L-N) derived from Human PLD2. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.<br/><br/>Steed P.M., FASEB J. 12:1309-1317(1998).<br />Lopez I., J. Biol. Chem. 273:12846-12852(1998).<br />Divecha N., EMBO J. 19:5440-5449(2000).<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr169 |
| Области исследований | Cancer,Cardiovascular,Metabolism,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Jurkat cells treated with TNF using PLD2 (Phospho-Tyr169) Antibody.The lane on the right is treated with the antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using PLD2 (Phospho-Tyr169) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Наименование: Phospho-PLD2 (Tyr169) Antibody.