| Объем | 100 мкл |
| Синонимы | ERK activator kinase 1/ERK activator kinase2, MAP kinase kinase 1/MAP kinase kinase 2, MAP2K1/MAP2K1, MAPK/ERK kinase 1/MAPK/ERK kinase 2, MAPKK 1/MAPKK 1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q02750 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 217/221 (I-D-S(p)-M-A) derived from Human MEK1/MEK2. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.<br/><br/>Shen R, et al. (2002) Mol Cell Biol, 22(10): 3230-3236<br/>Preisinger C, et al. (2005) EMBO J, 24(4): 753-765<br/>Laine P, et al. (2000) Biochem J, 349(Pt 1): 19-25<br/>Yaglom J, et al. (2003) Mol Cell Biol, 23(11): 3813-3824<br/>Dahan S, et al. (2002) Infect Immun, 70(5): 2304-2310<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser217/Ser221 |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cell untreated or treated with UV MEK1/MEK2(Phospho-Ser217/Ser221) Antibody
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1/MEK2(Phospho-Ser217/Ser221) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: Phospho-MAP2K1 (Ser217/Ser221) Antibody.