| Объем | 100 мкл |
| Синонимы | ASK-1, M3K5, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q99683 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 83 (G-S-S(p)-V-G) derived from Human ASK1. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death. <br /><br />Mabuchi S, et al. (2004) Endocrinology. 145(1): 49-58. <br />Yuan ZQ, et al. (2003) J Biol Chem. 278(26): 23432-23440. <br />Kim AH, et al. (2001) Mol Cell Biol. 21(3): 893-901.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser83 |
| Области исследований | Cardiovascular,Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from K562 cells using ASK1(Phospho-Ser83) Antibody(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1(Phospho-Ser83) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Western blot analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using ASK1 (Phospho-Ser83) Antibody.
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Наименование: Phospho-MAP3K5 (Ser83) Antibody.