| Объем | 100 мкл |
| Синонимы | NR1B1, RAR-alpha, Retinoic acid receptor alpha, retinoic acid receptor, alpha, RRA |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P10276 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 77 (P-P-S(p)-P-P) derived from Human Retinoic Acid Receptor alpha. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function By similarity. Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser77 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Metabolism,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Jurkat cells treated with PMA (125ng/ml, 30mins), using Retinoic Acid Receptor ?(Phospho-Ser77) antibody. The lane on the right is treated with the synthesized peptide.
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Western blot analysis of extracts from JK cells (Lane 2) and COS7 cells (Lane 3), using Retinoic Acid Receptor ? (Phospho-Ser77) Antibody. The lane on the left is treated with synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Retinoic Acid Receptor ? (Phospho-Ser77) antibody. The picture on the right is treated with the synthesized peptide.
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Наименование: Phospho-RARA (Ser77) Antibody.