Phospho-RB1 (Ser780) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | P105-RB, PP105, PP110, RB-1, RB1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P06400 | | Иммуноген | Peptide sequence around phosphorylation site of serine 780 (T-L-S(p)-P-I) derived from Human Rb. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 | | Примечание | Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.<br/><br/>Roesch A, et al.(2005) Mod Pathol. 18(4): 565-572.<br/>Huang H, et al.(2004)Oncogene. 23(12): 2161-2176.<br/>Kitagawa M, et al.(1996)EMBO J. 15(24): 7060-7069.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. | | Абревеатура | Phospho-Ser780 | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from 3T3 cells untreated or treated with UV using Rb(Phospho-Ser780) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Phospho-Ser780) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 | Immunofluorescence staining of methanol-fixed Hela cells using Rb(Phospho-Ser780) Antibody.
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 100 мкл | | Кат. номер: | CSB-PA133410 | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Phospho-RB1 (Ser780) Antibody.
Примечание: дополнительная информация (на английском языке). |
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