Phospho-AR (Ser650) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | ANDR, DHTR, AR | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P10275 | | Иммуноген | Peptide sequence around phosphorylation site of serine 650 (T-T-S(p)-P-T) derived from Human Androgen Receptor. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 | | Примечание | <p>The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. <br /><br />Brinkman, A.O. et al. (1999) J. Steroid. Biochem. Mol. Biol. 69, 307-313. <br />Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol. 76, 135-142. <br />Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146.</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. | | Абревеатура | Phospho-Ser650 | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Developmental biology,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Androgen Receptor (Phospho-Ser650) Antibody (left) or the same antibody preincubated with blocking peptide (right).
 | Western blot analysis of extracts from 293 cells, treated with PMA or calf intestinal phosphatase (CIP), using Androgen Receptor (Phospho-Ser650) Antibody.
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Наименование: Phospho-AR (Ser650) Antibody.