Объем | 100 мкл |
Синонимы | serum response factor |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P11831 |
Иммуноген | Peptide sequence around phosphorylation site of serine 77 (L-Y-S(p)-G-S) derived from Human SRF. |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC,WB:1:500-1:3000, IHC:1:50-1:100 |
Примечание | SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Абревеатура | Phospho-Ser77 |
Области исследований | Epigenetics and Nuclear Signaling,Cardiovascular,Developmental biology,Stem cells |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using SRF (Phospho-Ser77) antibody. The lane on the right is treated with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using SRF (Phospho-Ser77) antibody. The picture on the right is treated with the synthesized peptide.
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