Объем | 100 мкл |
Синонимы | APRF, Acute-phase response factor, HIES |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P40763 |
Иммуноген | Peptide sequence around phosphorylation site of tyrosine 705 (A-P-Y(p)-L-K) derived from Human STAT3. |
Источник | Rabbit |
Видовая специфичность | Human, Mouse, Rat |
Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
Примечание | <p>Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. <br /><br />Fan G, et al. (2003) J Biol Chem. 278(52): 52432-52436. <br />Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178. <br />Welsh, et al. (1996) Trends Cell Biol. 6: 274-279. <br />Srivastava A K, et al. (1998) Mol Cell Biochem. 182: 135-141.</p> |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Абревеатура | Phospho-Tyr705 |
Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cardiovascular,Developmental biology,Signal transduction,Stem cells |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells untreated or treated with IFN a using STAT3(Phospho-Tyr705) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (Phospho-Tyr705) Antibody (left) or the same antibody preincubated with blocking peptide (right).
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Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using STAT3 (Phospho-Tyr705) Antibody.
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Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with IFN using STAT3 (Phospho-Tyr705) Antibody.
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