| Объем | 100 мкл |
| Синонимы | Signal transduction protein CBL, Proto-oncogene c-CBL, Casitas B-lineage lymphoma proto-oncogene, RING finger protein 55, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P22681 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 770 (T-E-Y(p)-M-T) derived from Human c-Cbl. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.<br/><br/>Blake, T.J. et al. (1991) Oncogene 6, 653-657.<br/>Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473-482.<br/>Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339.<br/>Hunter, C. et al. (1999) J. Biol. Chem. 274, 2097-2106.<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr700 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HT29 cells untreated or treated with UV using c-Cbl(phospho-Tyr700) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Cbl(Phospho-Tyr700) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using c-Cbl(phospho-Tyr700) Antibody.
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Наименование: Phospho-CBL (Tyr700) Antibody.