Phospho-CDK2 (Thr14) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | CDC28, CDC2A, CDK1, MPF, kinase Cdc2 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P24941 | | Иммуноген | Peptide sequence around phospho rylation site of threonine14(E-G-T(p)-Y-G) derived from Human CDK1/CDC2 . | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB,WB:1:500-1:1000 | | Примечание | <p>Serine/threonine-protein kinase involved in the control of the cell cycle, essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression, controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus.<br/><br/>Sarkar S, Dey BK, Dutta A (2010) Mol Biol Cell 21, 2138-49 <br />Shreeram S, Hee WK, Bulavin DV (2008) Mol Cell Biol 28, 7442-50 <br />Mayya V, et al. (2006) Mol Cell Proteomics 5, 1146-57 </p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. | | Абревеатура | Phospho-Thr14 | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from HepG2 cells treated with Forskolin using Phospho-CDK1/CDC2 (Thr14) antibody.The lane on the right is treated with the antigen-specific peptide.
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 100 мкл | | Кат. номер: | CSB-PA228346 | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Phospho-CDK2 (Thr14) Antibody.
Примечание: дополнительная информация (на английском языке). |
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