| Объем | 100 мкл |
| Синонимы | MACS, MARCS, PKCSL, PRKCSL, Protein kinase C substrate |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P29966 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 158 (R-F-S(p)-F-K) derived from Human MARCKS. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IF,WB:1:500-1:1000, IF:1:100-1:200 |
| Примечание | MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.<br/><br/>Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30, 102(35): 12407-12412<br/>Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26, 280(3): 605-609<br/>Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15, 359(2): 151-159<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser158 |
| Области исследований | Cardiovascular,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 3T3 cells using MARCKS(Phospho-Ser158) Antibody(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
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Immunofluorescence staining of methanol-fixed Hela cells using MARCKS(Phospho-Ser158) Antibody.
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Наименование: Phospho-MARCKS (Ser158) Antibody.