| Объем | 100 мкл |
| Синонимы | Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64, MYC, BHLHE39 |
| Тип антител | Recombinant Antibody |
| Species | Human |
| UniProt ID | P01106 |
| Иммуноген | A synthesized peptide derived from human Phospho-MYC (S62) |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200 |
| Клональность | Monoclonal |
| Изотип | Rabbit IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Affinity-chromatography |
| Области исследований | Epigenetics and Nuclear Signaling |
| Аббревиатура | Myc proto-oncogene protein |
| Примечание | Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000). |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in:Hela whole cell lysate
All lanes:Phospho-MYC antibody at 1.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57 KDa
Observed band size: 57 KDa
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Immunofluorescence staining of Hela cells with CSB-RA015270A62phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Наименование: Phospho-MYC (S62) Antibody.