| Объем | 100 мкл |
| Синонимы | I-kappa-B-beta, IKB-B, IKBB, NF-kappa-BIB, NF-kappaB inhibitor beta |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q15653 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 23 (L-G-S(p)-L-G) derived from Human IkB-b. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation. <br /><br />Shirane, M. et al. (1999) J Biol Chem 274, 28169-28174. <br />DiDonato J, et al. (1996) Mol Cell Biol 16(4): 1295-304</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser23 |
| Области исследований | Epigenetics and Nuclear Signaling,Immunology,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-b(Phospho-Ser23) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 |
Western blot analysis of extracts from HT29 cells, treated with CA or calf intestinal phosphatase (CIP), using I?B-? (Phospho-Ser23) Antibody.
 |
| | |
Наименование: Phospho-NFKBIB (Ser23) Antibody.