| Объем | 100 мкл |
| Синонимы | Amplaxin, CTTN, EMS1, SRC8, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q14247 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 466 (P-V-Y(p)-E-T) derived from Human CORTACTIN. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB,WB:1:500-1:1000 |
| Примечание | <p>Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. <br /><br />Luo C, et al. (2006) J Biol Chem , 281(40):30081-30093 <br />Head JA, et al. (2003) Mol Biol Cell ,14(8):3216-3229 <br />Li Y, et al. (2000) J Biol Chem , 275(47): 37187-37193 <br />Kinley AW, et al. (2003) Curr Biol , 13(5): 384-393</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr466 |
| Области исследований | Cancer,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Jurkat cells untreated or treated with H2O2 using Cortactin(Phospho-Tyr466) Antibody.
 |
Western blot analysis of extracts from JK cells, treated with H2O2 or calf intestinal phosphatase (CIP), using Cortactin (Phospho-Tyr466) Antibody.
 |
| | |
Наименование: Phospho-CTTN (Tyr466) Antibody.