| Объем | 100 мкл |
| Синонимы | PIM-1, Proto-oncogene serine/threonine-protein kinase pim-1, EC 2.7.11.1, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P06803 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 309(A-A-V(p)-W-S) derived from Human Pim-1. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB,WB:1:500-1:1000 |
| Примечание | <p>Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. <br/><br/>Selten G., Cell 46:603-611(1986).<br />Saris C.J., EMBO J. 10:655-664(1991).<br />Maita H., Eur. J. Biochem. 267:5168-5178(2000).<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr309 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Signal transduction,Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HUVEC cells treated with PMA using Pim-1 (Phospho-Tyr309) Antibody.The lane on the right is treated with the antigen-specific peptide.
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Наименование: Phospho-Pim1 (Tyr309) Antibody.