| Объем | 100 мкл |
| Синонимы | DNPK1, PRKD, PRKDC, XRCC7, P460 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P78527 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine 2609 (V-E-T(p)-Q-A) derived from Human DNA-PK. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB,WB:1:500-1:1000 |
| Примечание | The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity, PRKDC must be bound to DNA to express its catalytic properties<br/><br/>Chan DW, et al. (2002) Genes Dev. Sep 15, 16(18): 2333-2338<br/>Ding Q, et al. (2003) Mol Cell Biol. Aug, 23(16): 5836-5848<br/>Douglas P, et al. (2002) Biochem J. Nov 15, 368(Pt 1): 243-251<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr2609 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from K562 cells untreated(lane 1) or treated with hydroxyurea(lane 2) using DNA PKcs(Phospho-Thr2609) antibody.
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Наименование: Phospho-PRKDC (Thr2609) Antibody.