| Объем | 100 мкл |
| Синонимы | Eukaryotic translation initiation factor 2 subunit alpha |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P05198 |
| Иммуноген | Peptide sequence around phosphorylation site of Serine 49 (L-L-S(p)-E-L) derived from Human eIF2a. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. <br /><br />Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29. <br />De Haro, C. et al. (1996) FASEB J. 10, 1378-1387. <br />Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128. <br />Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser49 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells untreated or treated with starvation using eIF2a(phospho-Ser49) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2? (Phospho-Ser49) Antibody (left) or the same antibody preincubated with blocking peptide (right).
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Immunofluorescence staining of methanol-fixed Hela cells using eIF2a(phospho-Ser49) Antibody.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using eIF2? (Phospho-Ser49) Antibody.
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Наименование: Phospho-EIF2S1 (Ser49) Antibody.