| Объем | 100 мкл |
| Синонимы | ELK1, ETS-domain protein Elk-1, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P19419 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 389 (P-R-S(p)-P-A) derived from Human Elk-1. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene <br /><br />Janknecht R, et al. (1993) EMBO J. 12(13): 5097-5104. <br />Marais R, et al. (1993) Cell 73:381-393. <br />Kortenjann M, et al. (1994) Mol Cell Biol. 14:4815-4824. <br />Hill C S, et al. (1995) Cell. 80:199-211. <br />Cavigelli M, et al. (1995) EMBO J. 14:5957-5964.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser389 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using Elk1(Phospho-Ser389) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1(Phospho-Ser389) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: Phospho-ELK1 (Ser389) Antibody.