| Объем | 100 мкл |
| Синонимы | MORT1 , FAS-associating death domain-containing protein, Mediator of receptor induced toxicity, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13158 |
| Иммуноген | Peptide sequence around phosphorylation site of Serine191(R-S-G(p)-A-M) derived from Human FADD. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
| Примечание | <p>The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.<br/><br/>Sugano S., Nat. Genet. 36:40-45(2004).<br />Farmer A., Submitted (MAY-2003).<br />Venter J.C., Submitted (JUL-2005).<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser191 |
| Области исследований | Cancer,Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Jurkat cells treated with PMA using FADD (Phospho-Ser190) Antibody.The lane on the right is treated with the antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using FADD (Phospho-Ser190) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Наименование: Phospho-FADD (Ser191) Antibody.