| Объем | 100 мкг |
| Синонимы | Ran-binding protein 10 (RanBP10), RANBP10, KIAA1464 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q6VN20 |
| Иммуноген | Recombinant Human Ran-binding protein 10 protein (3-165AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IP:1:200-1:2000 |
| Примечание | Acts as a guanine nucleotide exchange factor (GEF) for RAN GTPase (By similarity). May play an essential role in hemostasis and in maintaining microtubule dynamics with respect to both platelet shape and function (By similarity). May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation. In contrast to RANBP9, does not interact with Sos and does not activate the Ras pathway. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Ran-binding protein 10 |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate
All lanes: RANBP10 antibody at 2.1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68, 59, 65 kDa
Observed band size: 68 kDa
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IHC image of CSB-PA764955LA01HU diluted at 1:200 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunoprecipitating RANBP10 in Rat Brain tissue
Lane 1: Rabbit control IgG instead of CSB-PA764955LA01HU in Rat Brain tissue.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA764955LA01HU (6µg) + Rat Brain tissue (1mg)
Lane 3: Rat Brain tissue (20µg)
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Наименование: RANBP10 Antibody.