Объем | 100 мкл |
Синонимы | BBP, CW17, SF01, ZFM1, ZNF162 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q15637 |
Иммуноген | Peptide sequence around phosphorylation site of Serine 82(S-P-S(p)-P-E) derived from Human SF1. |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:100 |
Примечание | <p>Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor. <br/><br/>Susan Y. Park, Development, May 2005, 132: 2415 - 2423. <br />Dujin Zhou, Mol. Endocrinol., Jul 2000, 14: 986. <br />Dagmar Wilhelm, Genes & Dev., Jul 2002, 16: 1839. <br />Philipp Gut,Development, Oct 2005, 132: 4611 - 4619.<br /></p> |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Абревеатура | Phospho-Ser82 |
Области исследований | Epigenetics and Nuclear Signaling |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HUVEC cells treated with anisomycin using SF1 (Phospho-Ser82) Antibody.The lane on the right is treated with the antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using SF1 (Phospho-Ser82) antibody (left)or the same antibody preincubated with blocking peptide (right).
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