| Объем | 100 мкл |
| Синонимы | ROD, AUF1, HNRPD, |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q14103 |
| Иммуноген | Peptide sequence around phosphorylation site of Serine 83(N-S-S(p)-P-R) derived from Human hnRPD . |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. <br/><br/>Kajita Y., J. Biol. Chem. 270:22167-22175(1995).<br />Dempsey L.A., Genomics 49:378-384(1998).<br />Tolnay M., Biochem. J. 338:417-425(1999).<br />Grosset C., Cell 103:29-40(2000)<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser83 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from JK cells (Lane 2), using hnRPD (Phospho-Ser83) Antibody. The lane on the left is treated with antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using hnRPD (Phospho-Ser83) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Immunofluorescence staining of methanol-fixed HeLa cells using hnRPD (Phospho-Ser83) Antibody.
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Наименование: Phospho-HNRNPD (Ser83) Antibody.