| Объем | 100 мкл |
| Синонимы | MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MEK3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P46734 |
| Иммуноген | Peptide sequence around phosphorylation site of serine 189 (V-D-S(p)-V-A) derived from Human MKK3. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. <br /><br />Wang W, et al. (2002) Mol Cell Biol , 22(10): 3389-403. <br />Raingeaud J, et al. (1996) Mol Cell Biol, 16(3): 1247-55.</p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Ser189 |
| Области исследований | Epigenetics and Nuclear Signaling,Cancer,Immunology,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells untreated or treated with PMA using MKK3(Phospho-Ser189) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3(Phospho-Ser189) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using MKK3(Phospho-Ser189) Antibody.
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Western blot analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using MKK3 (Phospho-Ser189) Antibody.
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Наименование: Phospho-MAP2K3 (Ser189) Antibody.