RB1 (Ab-608) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | P105-RB, PP105, PP110, RB-1, RB1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P06400 | | Иммуноген | Synthesized non-phosphopeptide derived from human Retinoblastoma around the phosphorylation site of serine 608 (Y-L-S(p)-P-V). | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB,WB:1:500-1:3000 | | Примечание | Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex By similarity. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.<br/><br/>Lee W.-H., Nature 329:642-645(1987).<br/>Lee W.-H., Science 235:1394-1399(1987).<br/>Friend S.H., Proc. Natl. Acad. Sci. U.S.A. 84:9059-9063(1987).<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using Retinoblastoma (Ab-608) antibody.
 | Western blot analysis of extracts from JK cells (Lane 2), using Retinoblastoma (Ab-608) antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: RB1 (Ab-608) Antibody.