Объем | 100 мкл |
Синонимы | EC 2.7.11.24, Extracellular signal-regulated kinase 8 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q8TD08 |
Иммуноген | Peptide sequence around phosphorylation site of threonine 175 and tyrosine 177 (A-V-T(p)-E-Y(p)-V-A) derived from Human ERK8. |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB,WB:1:500-1:3000 |
Примечание | In vitro, phosphorylates MBP. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Абревеатура | Phospho-Thr175/Tyr177 |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Jurkat cells, using ERK8 (Phospho-Thr175+Tyr177) antibody. The lane on the right is treated with the synthesized peptide.
|
| |