| Объем | 100 мкл |
| Синонимы | MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPK2, MAPKAP kinase 2, MAPKAPK-2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P49137 |
| Иммуноген | Peptide sequence around phosphorylation site of threonine 334 (P-Q-T(p)-P-L) derived from Human MAPKAPK-2. |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | MAPKAPK-2 encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.<br/><br/>Rouse, J. et al. (1994) Cell 78, 1027-1037.<br/>Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.<br/><br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Thr334 |
| Области исследований | Cardiovascular,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using MAPKAPK-2(Phospho-Thr334) Antibody.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MAPKAPK-2(Phospho-Thr334) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Immunofluorescence staining of methanol-fixed Hela cells using MAPKAPK-2(Phospho-Thr334) Antibody.
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Наименование: Phospho-MAPKAPK2 (Thr334) Antibody.