| Объем | 100 мкл |
| Синонимы | PUM, EMA, H23AG, MUC-1, PEM |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P15941 |
| Иммуноген | Peptide sequence around phosphorylation site of tyrosine 1229 S-P-Y(p)-E-K) derived from Human CD227/MUC1. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Примечание | <p>The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. <br/><br/>Lan M.S., J. Biol. Chem. 265:15294-15299(1990).<br />Ligtenberg M.J.L., J. Biol. Chem. 265:5573-5578(1990).<br />Gendler S.J., J. Biol. Chem. 265:15286-15293(1990)<br /></p> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Абревеатура | Phospho-Tyr1229 |
| Области исследований | Cancer,Tags & Cell Markers,Immunology,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from HepG2 cells treated with PMA using CD227/MUC1 (Phospho-Tyr1229) Antibody.The lane on the right is treated with the antigen-specific peptide.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody (left)or the same antibody preincubated with blocking peptide (right).
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Immunofluorescence staining of methanol-fixed HepG2 cells using CD227/MUC1 (Phospho-Tyr1229) Antibody.
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Наименование: Phospho-MUC1 (Tyr1229) Antibody.